Around 100 female riders, including Olympians Laura Trott, Dani King and Jo Rowsell, as well as Wyman, will compete on a special 1.3-mile street circuit, known as a criterium, around Buckingham Palace and St James's Park.
The men will be in action on Sunday, in the 140-mile Surrey Classic (12:45 BST), which will feature around 150 of the world's top male cyclists.
Trott keen on increased coverage
Slovakia's Peter Sagan, who beat Mark Cavendish to win the green jersey for the points classification at the Tour de France, will take part, as will British veteran rider David Millar.
The festival opens with an eight-mile ride through central London, where roads will be closed for up to 50,000 people to take part.
Speaking to BBC Sport, Wyman, 32, added: "It's unfortunate, it's a shame that they have a criterium (a short circular race) for the women but a really impressive road race for the men.
Women's Tour de France
Began in 1984 as the 'Tour de France Feminin' but stopped after the 1989 event
Returned in 1992 - but after a legal battle over trademark rights, was forced to re-brand as the 'Grande Boucle' in 1998
Cancelled in 2004 because of logistics problems
Returned in 2005, won twice by Welsh cyclist Nicole Cooke before Pooley won the last event in 2009
"Cycling became really big in the last two or three years because of what Bradley Wiggins and Lizzie Armitstead have done. People who are new to the sport don't see inequalities so why enforce these inequalities by not treating men and women as equal?
"It's reinforcing the idea that women's cycling is not really a professional sport when it genuinely is. It's really sad."
Event director, Hugh Brasher, said: "The logistics and challenge of putting on the largest inaugural mass participation cycling event in the world has been huge. The complexities of getting 20,000 riders around a 100-mile course that four-and-a-half hours later has a UCI ranked professional race chasing them down, means that without even further road closures it would be logistically impossible to fit in another race.
"This is the first year of the event and we believe that have made an excellent start. More than 23% of the riders in the Prudential RideLondon-Surrey 100 are women. That is the highest percentage of women riders in a [sporting event] of this nature ever.
"The elite women's criterium race is the greatest field of its kind ever to race in the UK and we have 14 world champions, four Olympic and five Paralympic champions competing over the weekend."
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