And then there was one.
FC United of Manchester play host to League One side Chesterfield on Monday, in the last of the 40 first-round ties.
Can the club that was formed in 2005 reach the FA Cup second round for the second time in their history or will the Spireites avoid going the same way as Notts County, Barnsley and Bristol Rovers?
Join us for live text commentary ahead of Monday's 19:45 GMT kick-off.
Before then, be sure to catch our 90-minute highlights programme on BBC Two and online at 18:30 GMT.
It's been another day to remember - a day when it was proved anything can happen in the FA Cup.