Wednesday's preview performances of the Spider-Man Broadway musical were called off after a stunt man injured his back falling 30ft into a stage pit.
Instead, the cast and crew rehearsed new safety measures agreed with state officials who have now allowed the show to reopen on Thursday.
Spider-Man double Christopher W Tierney is expected to make a full recovery.
The official opening night has been pushed back to 7 February after a series of glitches and injuries.
Mr Tierney was injured in Monday night's performance of Spider-Man: Turn Off The Dark after falling despite wearing a safety harness that should have prevented such an accident.
His brother, Patrick, said the stunt man was due to have surgery on his back on Wednesday and would be released from hospital at the weekend.
New safety precautions include a requirement that a second person checks harnesses after they have been put on.
The show - which features 38 separate moves in which actors are put in harnesses to take to the air - has been beset with problems, including a lead actor suffering injury during a preview show.
Natalie Mendoza, who plays "spider goddess" Arachne, was hit in the head by a rope and suffered concussion during the first preview performance on 28 November.
Other injuries have included two broken wrists.
The $65m (£42m) production - the most expensive ever staged on Broadway - features music written by Bono and The Edge, of U2.
It has a 41-member cast, 18 orchestra members and has several complicated sets.