Up to 150 fans staged a march through Plymouth in protest at the failure of the club's directors to inject a £2m rescue package.
The cash-strapped League One club has survived several High Court winding-up actions and has not been able to pay players and staff on time.
Protesters met at the Big Screen in Armada Way before marching to Home Park for the Tranmere game.
Organiser Lee Rapson said the board had a "moral obligation" to save the club.
Fans' groups have called on supporters to boost attendances until the end of the season in a bid to raise at least £500,000.
An appeal to the city council to buy back Home Park and lease it to the club was rejected by the authority on cost grounds.
Council leader Vivien Pengelly said it was up to the board of directors to save the club.
Plymouth Argyle's short-term survival was secured earlier in the week when a winding-up petition from HM Revenue and Customs was dismissed in the High Court.
The League One club revealed it had paid off a £760,000 tax bill.