The rail line that runs into Wembley Stadium station will be shut on the day England play Croatia later this month.
Preparatory work for the new HS2 line will mean Chiltern Railway services will not run between Gerrards Cross and Wembley on 18 November.
The closure could leave thousands of fans from areas between Birmingham and London looking for alternative routes.
Network Rail said the work had been organised before the match had been scheduled and it could not be altered.
Check before travel on Sunday 18 November— Chiltern Main Line (@NetworkRailCML) November 1, 2018
Work to prepare for the new high speed rail line means no trains on the West Ruislip line for @chilternrailway
See @chilternrailway or @nationalrailenq for more info pic.twitter.com/Xb1MIiK5is
It said the date had been chosen to avoid interruption with Armistice Day remembrance events the weekend before.
Antonia Buckland, Network Rail's HS2 sponsorship director, said the organisation was "working closely with Chiltern Railways to minimise the impact".
The Chiltern line runs services from Birmingham to London Marylebone, via stations including Leamington Spa, Warwick, Banbury and High Wycombe.
It also runs trains from Kidderminster, Oxford, Stratford-upon-Avon and Aylesbury to the capital.
Southbound trains on the day of the match will run as far as Gerrards Cross in Buckinghamshire, from where replacement bus services will run to West Ruislip or Hillingdon, to connect with London Underground services.
Chiltern trains will run north from Marylebone to Wembley Stadium. More information can be found here.
The station sits directly next to the ground and is used by many fans travelling to and from the national stadium.
The stadium has limited parking spaces in order to encourage people to travel to Wembley using public transport.
Chiltern Railways said people who plan to travel should "visit our website for information and advice".
The FA said it did not want to comment.