Two hundred people will work around the clock during Christmas to install a new railway bridge over the A46.
The new dual carriageway stretch of A46 being constructed needs to go under the railway at Bingham and the bridge will take the line over the road.
Although no train services run on the line on Christmas Day and Boxing Day, buses will replace trains between Grantham and Bingham stations on December 27 and 28.
The A46 will be unaffected by the work.
Geoff Bethel from the Highways Agency said: "Working on Christmas Day and Boxing Day and on the following two bank holidays is the perfect opportunity for us to get the job done with minimum disruption at this time of year when there are fewer rail services.
"Our staff will be working round the clock to get the bridge into position and I thank them for giving up their Christmas to do so."
After the railway line is closed at 2030 GMT on Christmas Eve the teams of contractors will begin to install the 1300 tonne bridge.
Contractors will work in 10 hour shifts 24 hours a day.
Rail services between Nottingham and Norwich and Nottingham and Skegness will be affected on Monday, 27 December and some early services between Nottingham and Skegness will be affected on Tuesday, 28 December.
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