The pedestrian tunnel under the River Tyne will be closed for three days as part of plans for a £6m refurbishment.
The tunnel will be shut from 0900 GMT and is due to reopen on Friday afternoon. Cyclists and pedestrians can use the adjacent cycle tunnel.
Tyne and Wear Integrated Transport Authority plans to replace two escalators in the grade ll listed tunnels with lifts.
The closure will allow investigation work under the floor of the tunnel.
There are plans to removed two of the wooden escalators and replace them with incline lifts.
Two of the escalators will be kept because of their historical significance.
Authority project director Paul Fenwick said: "We are well on the way to submitting an application for Listed Building Consent to the two local planning authorities of North Tyneside and South Tyneside to enable the major refurbishment works to go ahead.
"The closure of the pedestrian tunnel will give us the opportunity to look closely at the structure and inform our plans for the refurbishment."