The NFL will introduce a rule to require teams to interview female candidates for "executive positions".
It follows the Rooney Rule, established in 2003, which requires NFL teams to interview at least one minority candidate for head coaching or senior football operation jobs.
Jen Welter was a member of the Arizona Cardinals coaching staff in pre-season, while Kathryn Smith recently became the first full-time female coach with an NFL team when she was hired by the Buffalo Bills.
At boardroom level, Katie Blackburn is the long-serving executive vice-president of the Cincinnati Bengals, while Amy Trask was the Oakland Raiders' chief executive officer from 1997 until 2013.
There is also a female game official - Sarah Thomas - in the NFL.
NFL commissioner Roger Goodell, speaking at the league's Women's Summit in the build-up to Super Bowl 50, said: "We believe in diversity. We believe that we're better as an organisation when we have good people at the table."