American Justin Gatlin improved on his own world-leading 100m time, clocking 9.86 seconds at the Golden Spike meet in the Czech Republic.
The 32-year-old, who won Olympic gold in Athens in 2004, now has the four fastest times of 2014 to his name.
In Ostrava, he surged clear from the outset and improved on the 9.87secs he ran in Beijing last month, despite a head wind of 0.4 metres per second.
Mike Rodgers was second in 10.08 ahead of veteran Kim Collins in 10.12.
There were impressive performances from British athletes as 23-year-old Niall Flannery won the 400m hurdles in a new personal best of 48.80 secs, which puts him top of the British and European rankings for 2014.
Bianca Williams, 20, also set a new personal best of 22.79 secs finishing second in the 200m, just 0.04 behind 2012 Olympic champion Allyson Felix, while Williams took a further 0.14 off her best time set earlier this year.
Lawrence Clarke, 24, won the 110m hurdles in 13.50 secs, Martyn Rooney was third in the 400m in 45.03 secs, while, in the 100m, Harry Aikines-Aryeetey and Sean Safo-Antwi were fifth and sixth respectively in 10.19 and 10.21 secs.