World indoor champion Richard Kilty claimed the starting gun made no sound at the start of his 100m race at the Anniversary Games in London.
Kilty pulled up thinking there had been a false start, but the race continued and he trailed in last.
"It was obvious, ask the other competitors, nobody heard the gun," said Kilty, 24.
Harry Aikines-Aryeetey, Kilty's England team-mate in Glasgow, won in 10.29 seconds.
Kilty, who announced his arrival on the global sprint scene by winning 60m gold at the World Indoor Championships in March, added: "This run was meant to be a confidence booster for the Commonwealths and through no fault of my own it's messed me up a little bit."
England's Will Sharman impressed in the 110m hurdles, narrowly losing a three-way photo finish.
The 29-year-old finished third in 13.24 seconds behind American Ryan Wilson and Shane Brathwaite from Barbados, with all three athletes given the same time.
England's Tiffany Porter beat Australian rival Sally Pearson in the 100m hurdles.
Chris Tomlinson leaped 7.94m to win the long jump with Olympic champion Greg Rutherford sidelined by a knee injury, while American Allyson Felix beat Jamaican rival and two-time Olympic champion Shelly-Ann Fraser-Pryce in the 100m.
The Anniversary Games mark two years since the London Olympics and Paralympics, and 2012 Paralympic T44 100m champion Jonnie Peacock again came off second best in his tussle with American world record holder Richard Browne, who also beat him in Glasgow last weekend.
There were victories for Paralympic champions Hannah Cockroft in the T34 100m and Aled Davies in the F42 shot put, London 2012 silver medallist Stef Reid in the F42/44 long jump, and world champion Sophie Hahn in the T37/38 100m.
Out on The Mall - the scene of his six London Marathon and London Paralympic marathon triumphs - wheelchair racer David Weir enjoyed more success, winning the T53/54 mile in 3:20.00.