Ireland's Katie Taylor will put her status as undisputed world lightweight champion on the line against decorated Puerto Rican Amanda Serrano in Manchester on 2 May.
Serrano, 31, has won world titles in seven weight divisions.
"It's a fight that seems to have been talked about for years so I'm just glad it's finally on," said Taylor, 33.
Taylor edged Belgium's Delfine Persoon in a close bout to become undisputed lightweight champion in June 2019.
Many observers felt Persoon had won the contest but Taylor got the decision to add the WBC lightweight belt to her IBF, WBO and WBA titles.
The Irishwoman then became a two-weight world champion in November when outpointing Greece's Christina Linardatou in Manchester to land the WBO light-welterweight belt and a 15th straight professional win.
Serrano has earned world titles at super-flyweight, bantamweight, super-bantamweight, featherweight, super-featherweight, lightweight and light-welterweight, and boasts a 24-fight winning streak.
"I'm happy we finally got this big fight made because both myself and Katie deserve to be in this position," said Serrano, who is based in New York, USA.
"While our fight could have been a main event, I'm still grateful it's happening.
"I'm not going to talk down to Katie, as I know she won't to me, so I'll leave it at this: one thing that's for sure is that we'll both leave it all in the ring."
Taylor's promoter Eddie Hearn said the bout would be "the biggest women's fight of all time".
The fight will see Taylor returning to the Manchester Arena, where she beat Linardatou.