Former Tour de France winner Andy Schleck is out of this year's race, saying he has no chance of being fit in time for the 30 June start.
The RadioShack-Nissan rider, 27, fractured his pelvis in a crash during the Criterium du Dauphine last week.
"I'm not going to be in [the Tour]," Schleck said. "I will need to be back to 100% before I can [resume] riding."
Schleck, from Luxembourg, still hopes to be fit for London 2012 and the Vuelta (Tour of Spain) in August.
Scans following the accident on 7 June revealed the fracture.
He added: "Yesterday, I tried going riding for an hour in the rain but I realised straight away that it would be impossible.
"When I abandoned the Dauphine I was in pain, but that was even worse. After that, I knew that I could not go to the Tour."
Schleck, who has finished the Tour as runner-up three times, was awarded the 2010 title following the disqualification of Spain's Alberto Contador for doping offences.
This will be the first time Schleck, a major rival of Britain's Bradley Wiggins, has missed the race since making his debut in 2008 with CSC.