The UK Anti-Doping Agency (Ukad) is investigating claims Team Sky may have breached cycling's 'no needles' policy, according to the Press Association.
Governing body the UCI introduced the ban in 2011, but a whistleblower said Team Sky continued to inject riders.
One of the substances said to have been injected is Fluimucil, at the heart of the controversy over a mystery package delivered to Bradley Wiggins in 2011.
Team Sky had been renowned for their robust no-needle stance.
In March, British cyclist Josh Edmondson told the BBC he broke the sport's rules by secretly injecting himself with a cocktail of vitamins when riding for Team Sky.
The substances are not illegal under World Anti-Doping Agency (Wada) rules but there are concerns about the ethics of administering them intravenously.
Ukad and the UCU are investigating claims by Edmondson that Team Sky covered up his use of banned injections.
Team Sky deny doing so, insisting that Edmondson did not confess at the time, and that they did not report the case because of concerns about his mental wellbeing.
PA's source, who sent the information to the House of Commons' Culture, Media and Sport Select Committee and Ukad, is understood to have worked for Team Sky.
In an email to the committee, seen by the BBC, the source said some Team Sky doctors were using intravenous recovery methods towards the end of the team's difficult first season in 2010 and continued to do so despite the UCI ban, which Team Sky publicly supported.
The source has claimed Team Sky hired Dr Fabio Bartalucci in late 2010 as he was believed to have expertise in IV recovery.
The Italian, who was picked up by police in an anti-doping raid at the 2001 Giro d'Italia but not charged, left in 2011.
Ukad said it "does not discuss or disclose information in relation to its investigations".
Culture, Media and Sport Select Committee chairman Damian Collins said: "This raises further concerns about how Team Sky policed the use of medication for riders outside of competition, and how hard they were prepared to press up against the line of what was allowed or acceptable."
BBC Sport attempted to contact Team Sky. However, a spokesperson earlier told PA: "It is right that any concerns are reported to and dealt with by the appropriate authorities, and we will continue to co-operate with them."