Republic of Ireland striker Kevin Doyle has been ruled out of the Euro 2012 qualifier against Macedonia on 4 June.
The 27-year-old aggravated a knee injury after coming on as a Wolves substitute in Saturday's 3-1 win at Sunderland.
Doyle was returning to action after damaging medial ligaments in the Republic's victory over Macedonia in Dublin in March.
Fulham's Damien Duff will also miss the game because of an Achilles injury.
Republic manager Giovanni Trapattoni has fitness worries over Sean St Ledger and Darren O'Dea for the qualifier in Macedonia.
Doyle and Duff are also sidelined for the Carling Nations Cup games against Northern Ireland and Scotland in Dublin on 24 and 29 May.
The Republic beat Wales 3-0 in their opening Nations Cup game in Febuary.
Republic of Ireland squad: Given (Man City), Westwood (Coventry), Forde (Millwall); Dunne (Aston Villa), O'Shea (Man Utd), O'Dea (Ipswich), St Ledger (Preston), Foley (Wolves), Delaney (Ipswich), Clark (Aston Villa), Coleman (Everton), Kilbane (Huddersfield), Kelly (Fulham), McShane (Hull); McCarthy (Wigan), Whelan (Stoke), Wilson (Stoke), Gibson (Man Utd), Andrews (Blackburn), McGeady (Spartak Moscow), Fahey (Birmingham), Lawrence (Portsmouth), Treacy (Preston), Hunt (Wolves), Ward (Wolves); Keogh (Wolves), Stokes (Celtic), Folan (Colorado Rapids), Walters (Stoke), Keane (West Ham), Long (Reading).