AFC Totton's tie against Bristol Rovers will be one of three televised FA Cup matches, landing the non-league club a guaranteed windfall of £72,000.
Totton beat to reach the second round for the first time in their history.
The Southern Premier Division side's reward was a tie against League Two Rovers which will now be played on Sunday, 4 December at 1230 GMT.
The match will also be played at Totton's Testwood Stadium home.
Officials had met with local police and safety officials to see if their stadium could host the tie with the club getting the green light on Monday.
Totton's Testwood Stadium will host 2,375 fans for the clash with Rovers receiving an allocation of 245.
Tickets are expected to go on sale in the week commencing 21 November with priority given to season ticket holders, then members and then the general public.
The club have ruled out erecting a temporary stand for the match.
"The financial implications are massive," Totton manager Stuart Ritchie told BBC Radio Solent on Sunday.
The television revenue from the second round means the non-league will have earned over £100,000 from this year's competition in prize money and gate receipts.