Callum McManaman scored his first goal for in-form Blackpool as Reading suffered their first loss in three Championship matches.
McManaman, on loan from Wigan, beat Royals keeper Adam Federici with a curling shot shortly after half-time to hand the Tangerines victory.
Adam Le Fondre, Hal Robson-Kanu and Jimmy Kebe all went close for the Royals in the first half.
Ludovic Sylvestre and Lomana LuaLua had chances for Blackpool.
Both sides had claims for penalties turned down over the course of the match.
Blackpool appealed after they believed Shaun Cummings had fouled on McManaman while Reading thought Alex Pearce had been pushed in the area.
The visitors also had the ball in the back of the net with 10 minutes remaining of the opening period through Simon Church, but his effort was ruled out by an offside decision.
The victory moves Blackpool up to seventh in the Championship, two points adrift of Brighton.
Reading themselves would have moved up to seventh had they won, but they remain in mid-table with five points separating them from a play-off place and seven from the drop-zone.