Swindon boss Paolo di Canio says his side must maintain confidence by extending their unbeaten 12-game run.
Di Canio's men, unbeaten since the 2-0 loss to Macclesfield in September, now visit Southend in Tuesday's Johnstone's Paint Trophy southern semi-final.
"It is important to build the confidence up," he told BBC Wiltshire.
"It is important that, if we are 1-0 down or if we lose one game, you can identify there's a body and a soul in this team," added Di Canio.
After Matt Ritchie's goal snatched a 1-0 FA Cup second round victory at Colchester on Saturday to earn next month's third round date with Premier League side Wigan Athletic, Di Canio admits that result will help bolster belief.
"It is important that we don't lose that desire," he said. "One day we will lose, I hope far from now, but we know that we are a good team.
"We're not worried about any side in League One or in League Two."
Swindon have already turned over former Robins boss Paul Sturrock's Southend once this season, enjoying a 2-0 win over the Shrimpers at the County Ground back in September.
"We'll be playing against the team top of our league," said Di Canio, "but we have beaten them already and we know that we can beat any side.
"But it is important that we play with the commitment that we showed against Colchester."