Uefa's betting fraud detection system has not shown any irregular betting patterns around Lyon's 7-1 Champions League win over Dinamo Zagreb.
To reach the knockout stages, Lyon needed victory in Croatia, Group D rivals Ajax to lose to Real Madrid, and a seven-goal swing in goal difference.
Bafetimbi Gomis scored four times in the space of 25 minutes in Zagreb for Lyon, while Ajax were beaten 3-0.
But European football's governing body will not investigate at this stage.
Earlier on Thursday, Lyon backed the decision of the French online gaming authority (Arjel) to run checks on bets placed on the result.
The body later announced that "no particular anomaly" had been found during their probe.
French striker Gomis scored a hat-trick in seven minutes - shaving two minutes off the mark Mike Newell set for Blackburn against Rosenborg in 1995 for the fastest Champions League treble.
Gomis equalised on the stroke of half-time before scoring three times in the second half. Maxime Gonalons, Lisandro Lopez and Jimmy Briand also netted for the visitors.
Dinamo, who had a man sent off, issued a statement describing speculation about the result as "shameful, malicious and tendentious".
"[It's] a defeat that was hard to imagine," said the statement. "A team that was more powerful, superior in numbers and had greater motivation won yesterday."
Ajax coach Frank de Boer added: "If there was something unusual, Uefa should investigate what happened in Zagreb."