Goals from Ian Henderson, Tom Eastman, Freddie Sears and an own goal from Ian Goodison saw Colchester record a comfortable victory over Tranmere.
Henderson headed Colchester ahead before Eastman followed suit also nodding home but Tranmere pulled one back through James Wallis's low shot.
The U's restored their advantage when Rovers' Goodison diverted Michael Rose's corner into his own net.
Jake Cassidy pulled a goal back before Sears sealed it with a fine strike.
VIEWS FROM THE DRESSING ROOM
Colchester United manager John Ward told BBC Essex:
"For me, it's what we've deserved over the season, particularly over the last eight weeks where we've drawn too many games.
"At the same time we've had matches where we could have done what we've done today, found the net a little bit more.
"So I'm really pleased they've gone out on that high, it's been a nice reward for everybody today.
"It could have been 8-4 or 5, but we deserved to win the game, we had more opportunities to score."