Oxford City and AFC Totton are just one win away from promotion to the Blue Square Bet South, after winning their respective play-offs on Thursday.
Totton defeated Chesham 3-2 while Oxford City beat Cambridge City 1-0 to advance through to Monday's final.
"It's a massive game for both clubs and hopefully we'll get a big crowd and we are looking forward to it," Oxford City boss Mike Ford told BBC Radio Oxford.
Totton's Stuart Ritchie told BBC Radio Solent: "The pressure is all on them."
The match will take place at City's Court Farm Place after former Oxford United defender Chris Wilmott sealed victory against Cambridge City.
"We dug in when we had to, we created opportunities when we had to," added Ford.
"We are very resilient and weathered the storm at the end. We deserve to be in that final."
For Ritchie it will be his last game as manager with Totton's boss widely linked to the vacant job at Havant and Waterlooville.
Since taking over in 2004, Ritchie and his assistant Sean New have lead the Stags to two promotions so far, a Wembley appearance in the 2007 FA Vase final and a historic FA Cup run to the second round proper where they faced Bristol Rovers live on TV.
"It is a fantastic achievement and roll on Monday," added Totton boss Ritchie.
"We will go and enjoy it. I believe in this group and they can achieve great things. They really dig in and put their bodies on the line."