Midfielder Hayley Ladd was sent off during Wales' 2-0 defeat to hosts Portugal in their opening match of the Algarve Cup on Wednesday.
Ladd was sent off for a second yellow card and will be unavailable for Wales' next match against Mexico.
Portugal went in 1-0 ahead at half-time thanks to captain Edite Fernandes' 37th-minute penalty.
The striker struck again on 72 minutes to end Wales hopes despite an impressive second-half display.
Jarmo Matikainen's Wales side play Mexico, who beat Hungary 1-0, on Friday.
Wales Women: Nicola Davies (Unattached), Loren Dykes (capt, Bristol Academy), Sophie Ingle (Chelsea), Kylie Davies (Chelsea), Lauren Price (Cardiff City Ladies), Hayley Ladd (Coventry), Angharad James (Bristol Academy), Jessica Fishlock (Seattle Reign FC), Helen Ward (Chelsea), Sarah Wiltshire (Watford), Natasha Harding (Bristol Academy)
Subs: Rhian Nokes (Cardiff City Ladies), Nicola Cousins (Cardiff City Ladies), Carys Hawkins (Sunnana SK), Danielle Oates (Blackburn), Lauren Hancock (Cardiff City Ladies), Megan Wynne (Watford), Michelle Green (Cardiff City Ladies), Shan Jones (Caernarfon Ladies), Amy Thrupp (Pilcs), Rachel Hignett (Bristol Academy)