Queen's Park sustained their fourth defeat in five matches as second-bottom Annan Athletic claimed their first win in a month.
Blair Spittal and Michael Keenan had early chances for the visitors and the hosts threatened through Michael Daly.
Scott Chaplain swept past Neil Parry to give Annan a 28th-minute lead.
And, after home goalkeeper Alex Mitchell twice denied Aidan Connolly, David Hopkirk tucked home Daly's pass for a late second.
The Spiders remain second but are still not guaranteed a promotion play-off spot.
Annan Athletic: Mitchell, Chisholm (Monaghan 85), McNiff, Peter Watson, McGowan, Sloan, Jardine, Chaplain (Love 88), McKechnie, Daly, Hopkirk. Subs Not Used: Summersgill, Donley, Blake.
Booked: Daly, Sloan.
Goals: Chaplain 28, Hopkirk 84.
Queen's Park: Parry, Little, Gallacher, Bradley, Robertson, Shankland (Connolly 46), Keenan, Anderson, McParland (Urquhart 69), Longworth (Burns 46), Spittal. Subs Not Used: Lochhead, Quinn.
Ref: Gary Hilland