A Jamaican footballer failed a drugs test following a World Cup qualifier against Honduras on 11 June.
It is a further blow to sport in the country after sprinters Asafa Powell and Sherone Simpson tested positive for the banned stimulant oxilofrine last month.
The Jamaican Football Federation (JFF) has confirmed it had been informed by Fifa of the positive result.
An internal investigation has been opened by the JFF.
Jamaica lost the qualifier 2-0 in Honduras.