Barcelona left-back Jordi Alba has been ruled out for six weeks with a thigh injury.
The 24-year-old Spain international had been close to returning from a hamstring tear than has kept him out for almost a month.
Barca are top of the Primera Division after winning all eight games, ahead of Atletico Madrid on goal difference.
Coach Gerardo Martino has centre-backs Javier Mascherano, 29, and Carlos Puyol, 35, returning from injuries.
The pair came through a friendly against third tier side Sant Andreu unscathed.
Marc Bartra, 22, has been playing regularly in recent weeks.
But the Catalans defence is likely to have more experience when they face Osasuna - after the international break - on Saturday, 19 October.