Players from Brockenhurst and Andover Town set a new record as they scored 29 consecutive penalties in a shoot-out.
The Hampshire Senior Cup match finished 0-0 after extra time, so went to spot-kicks.
And after 29 successful attempts - a total confirmed by the Football Association as an English record - Andover's Claudio Herbert missed.
"I didn't realise the magnitude, it was a bit of a blur, the keeper pulled off a great save," Herbert told BBC Sport.
The sequence of successful attempts is also thought to be a world record.
The previous English record for the most consecutive penalties scored in a shoot-out was set when Dagenham & Redbridge defeated Leyton Orient in a Johnstone's Paint Trophy Tie in 2011 after 27 consecutive successful kicks.
The record for the longest penalty shoot-out came in 2005 when the Namibian Cup had to be settled by a record-breaking 48 spot-kicks, with KK Palace holding their nerve to defeat the Civics 17-16 following a 2-2 draw in normal time.
Herbert, 20, who is a full-time student, says he never dreamed he would need to take two penalties in the shoot-out but would have no hesitation stepping up and doing it all again.
"I was excited but nervous," he said. "I never thought I'd have to take the 30th penalty in a shoot-out.
"I've been involved in penalty shoot-outs before, but nothing like this. There was not so much pressure.
"I would definitely take another one. I'd never shy away from taking a penalty."
He says despite the miss his team-mates attached no blame to him, adding: "I was just thinking 'oh no, it's going to be on TV - I'm going to miss a penalty on TV'."
His manager at Andover, Neil Benson, said the defeat was hard to accept at the end of a game which began at 19.40 BST and ended at 23:00 BST.
"It was a tough one to take," Benson told BBC Sport. "It was with some disbelief after the first set of penalties that we had to do it all over again. Every credit to the 11 players from both sides."
Herbert had the unfortunate task of trying to level at 15-15, but the keeper pulled off an astonishing save.
"He is an excellent player and will continue to be confident with everything he does on the pitch," added Benson. "It won't affect him and given the opportunity he will step up and do it again."