Tranmere have suspended manager Ronnie Moore, pending the conclusion of a Football Association investigation into a potential breach of betting rules.
It is understood the allegation relates to regulations which prevent him from betting on any competition in which his club is involved.
The FA has neither confirmed nor denied that Moore was the subject of an investigation by its integrity unit.
The League One side have put assistant John McMahon in temporary charge.
Moore, 61, became manager of Rovers for a second time in 2012 following spells at Oldham, Southport and Rotherham.
Tranmere currently sit 18th in the table, two points clear of the relegation zone.