Cardiff City defender Declan John has been handed a first call-up to the Wales Under-21 squad for the Euro 2015 qualifier against England at Derby on 5 March.
John, 18, has played at Under-17 and Under-19 level and made his senior debut in the against Macedonia.
Southampton's Lloyd Isgrove is recalled to the squad.
Manchester City's Emyr Huws, on loan at Birmingham, has been called up to the senior squad for the Iceland friendly.
One change to the original squad sees Fulham's George Williams replacing Torquay United's Billy Bodin, who has withdrawn because of injury.
Geraint Williams' side are currently third in Group 1, six points behind group leaders England.
Williams is set to add to further players to the squad, a goalkeeper and outfield player.
Wales Under-21 squad: Connor Roberts (Cheltenham Town), Daniel Alfei (Swansea City - on loan at Portsmouth), Morgan Fox (Charlton Athletic), Adam Henley (Blackburn Rovers), Declan John (Cardiff City), George Ray (Crewe Alexandra), Joe Walsh (Crawley Town), George Williams (Fulham), Gwion Edwards (Swansea City), Lee Evans (Wolverhampton Wanderers), Lloyd Isgrove (Southampton), Lee Lucas (Swansea City - on loan at Cheltenham Town), Wes Burns ( Bristol City), Tom Lawrence (Manchester United on loan at Yeovil Town), Robert Ogleby (Wrexham), Tom O'Sullivan (Cardiff City)