Manchester United forward Juan Mata and Chelsea striker Fernando Torres have been left out of the Spain squad for next Wednesday's friendly with Italy in Madrid.
"This is just a squad to play Italy, not for the World Cup," said coach Vicentre del Bosque. "Some players not on this list have a lot of merit."
Atletico Madrid striker Diego Costa has been included and could make his debut for Spain.
Brazil-born Costa, who has scored 27 goals for Atletico this season, was called up for the friendlies against Equatorial Guinea and South Africa last November, but missed both with injury.
Spain squad to face Italy
Goalkeepers: Iker Casillas (Real Madrid), Victor Valdes (Barcelona), Pepe Reina (Napoli).
Defenders: Sergio Ramos (Real Madrid), Jordi Alba (Barcelona), Javi Martinez (Bayern Munich), Cesar Azpilicueta (Chelsea), Juanfran Torres (Atletico Madrid), Raul Albiol (Napoli).
Midfielders: Xavi Hernandez, Sergio Busquets, Andres Iniesta, Cesc Fabregas (all Barcelona), Xabi Alonso (Real Madrid), David Silva (Manchester City), Thiago Alcantara (Bayern Munich), Koke (Atletico Madrid), Santi Cazorla (Arsenal).
Forwards: Pedro Rodriguez (Barcelona), Jesus Navas, Alvaro Negredo (both Manchester City), Diego Costa (Atletico Madrid).