Manchester United midfielder Tom Lawrence has pulled out of the Wales Under-21 for the Euro 2015 qualifier against England next Monday.
The 20-year-old picked up an injury in United's Under-21 Premier League final defeat to Chelsea at Old Trafford.
Lawrence had scored to give United the lead but Chelsea came from behind to win 2-1.
The Wrexham-born player recently made his senior United debut in the 3-1 win over Hull City.
Lawrence has been replaced in the squad by Swansea City's James Loveridge while Scott Tancock has been replaced in the squad by Swans team-mate Danny Alfei.
Geraint Williams' side host Gareth Southgate's England at Swansea's Liberty Stadium on Monday, 19 May.
Wales, who lost 1-0 to group leaders England at Derby in March, are currently third - 19 points behind England and three points behind second-placed Moldova.
Monday's match in Swansea will be their final home game of the campaign with away matches in September away to Finland and Lithuania to complete the group.
Wales Under-21 squad: Connor Roberts (Cheltenham Town), Daniel Ward (Liverpool), Morgan Fox (Charlton Athletic), Kieron Freeman (Derby County on loan at Sheffield United), Joe Walsh (Crawley Town), Danny Alfei (Swansea City), Lee Lucas (Swansea City), Declan John (Cardiff City), George Ray (Crewe Alexandra), Tom O'Sullivan (Cardiff City), James Loveridge (Swansea City), Lloyd Isgrove (Southampton on loan at Peterborough United), Lee Evans (Wolverhampton Wanderers), Wes Burns (Bristol City), Rob Ogleby (Wrexham), Gwion Edwards (Swansea City on loan at Crawley Town), Josh Pritchard (Fulham), George Williams (Fulham)