Russia captain Roman Shirokov has been ruled out of the World Cup after failing to recover from a knee problem.
The Zenit St Petersburg midfielder, 32, picked up the injury in April and will have surgery in Finland next week.
Russia manager Fabio Capello said: "Shirokov is a very important member of Russia's squad but I hope that the rest of the players will be able to perform at their best level in Brazil."
Rubin Kazan midfielder Pavel Mogilevets will replace Shirokov in the squad.
Shirokov missed friendlies against Slovakia and Norway but was able to train with the rest of the squad on Thursday before suffering a relapse.
Capello, who took England to the last 16 in 2010, said his team would play one more warm-up match, against Sao Paulo state champions Ituano on 11 June.
Russia will open their World Cup campaign against South Korea on 18 June. They will also face Belgium and Algeria in Group H.
Revised Russia squad
Goalkeepers: Igor Akinfeev (CSKA Moscow), Yury Lodygin (Zenit St Petersburg), Sergey Ryzhikov (Rubin Kazan).
Defenders: Vasili Berezutskiy (CSKA Moscow), Vladimir Granat (Dynamo Moscow), Andrey Eshchenko (Anzhi Makhachkala), Sergey Ignashevich (CSKA Moscow), Alexey Kozlov (Dynamo Moscow), Dmitry Kombarov (Spartak Moscow), Andrey Semenov (Terek Grozny), Georgi Schennikov (CSKA Moscow).
Midfielders: Denis Glushakov (Spartak Moscow), Igor Denisov (Dynamo Moscow), Alan Dzagoev (CSKA Moscow), Yury Zhirkov (Dynamo Moscow), Alexey Ionov (Dynamo Moscow), Pavel Mogilevets (Rubin Kazan), Alexander Samedov (Lokomotiv Moscow), Victor Faizulin (Zenit St Petersburg), Oleg Shatov (Zenit St Petersburg).
Forwards: Maxim Kanunnikov (Amkar Perm), Alexander Kerzhakov (Zenit St Petersburg), Alexander Kokorin (Dynamo Moscow).
For the best of BBC Sport's in-depth content and analysis, go to our features and video page.