Kevin Wilkin is frustrated he will be without midfielder Dean Keates and striker Andy Bishop for Wrexham's first game of the Conference season at Dartford.
Captain Keates has a hamstring injury while Bishop is still struggling with a groin problem.
Midfielder Jay Harris is also doubtful with a hip injury for Saturday's game.
"All three of those are senior players and key players to what we can do this year," Wilkin said.
"Andy Bishop has had a nagging problem pretty much throughout the course of pre-season.
"Dean Keates came off against Rhyl so those two won't feature for us.
"There's a question mark over Jay Harris now, which is really disappointing.
"Jay will hopefully will be fit and hopefully it's nothing more than a niggle.
"It's something we need to address and we need to make sure that these niggles are dealt with and don't end up being longer term problems."