Striker Wilfried Bony has replaced Stevan Jovetic in Manchester City's squad for the Champions League.
Ivory Coast international Bony, 26, joined City from Swansea City last month in a £25m deal.
His arrival meant City had one more foreign player in their squad than they are permitted to register for European competition.
City face Barcelona in the last 16 of the competition - the first leg of which is at home on 24 February.
The return leg at the Nou Camp is three weeks later.
The decision by manager Manuel Pellegrini to leave out Jovetic, 25, means he will continue to be restricted to just three senior strikers for the remainder of the competition - Bony, Sergio Aguero and Edin Dzeko.
City, as punishment for breaching Uefa's Financial Fair Play restrictions, are restricted to naming just 16 foreign players in their squad rather than the usual 17 with their squad capped at 21.
France full-back Gael Clichy and Belgium defender Dedryck Boyata do not count on the foreign quota as they qualify as home-grown and club-trained players respectively.
Jovetic, who missed large spells last season with calf and hamstring problems, has scored just 11 goals since his £22m move from Fiorentina in 2013.