Manager Gary Locke urged his Kilmarnock players to stop leaking goals if they want to guarantee their Scottish Premiership safety.
Leading 1-0 against Hamilton at Rugby Park through a Lee Ashcroft header, Killie then lost three in succession to Accies before Greg Kiltie pulled one back in a 3-2 defeat.
It means the Ayrshire side have lost six league games in a row, indeed every match since Locke was awarded a three-year contract.
"We went a goal up and I thought that would have given us the impetus to go on and do well," the manager told BBC Scotland.
"Hamilton finished the first half strongly, kept the ball well and in the second half the goals we lost were disappointing.
"We were an attacking threat today but we've got to make sure we stop losing sloppy goals.
"They took their chances and we didn't. They are a good side and they pass the ball well.
"We're really disappointed at not getting anything out of the game."
Killie's next game is away to second-bottom Motherwell, who are six points behind Locke's side and Ross County with three games to go.
The team finishing second bottom goes into a Premiership play-off final against Hibernian, Rangers or Queen of the South, the Championship teams who made the play-offs.
"When you're on the run that we've been on the confidence is a bit lower than normal," added Locke.
"But we've not got time to feel sorry for ourselves, we've got to pick ourselves up. We've got another big game next Friday and we need to make sure we get something out of that.
"Motherwell are fighting for their lives and we're the same so it's a game that the players should want to be involved in."
Hamilton manager Martin Canning was delighted to follow a win over Motherwell in their last game with another victory.
He said: "It was a great three points for us. Coming from behind shows the character and togetherness of the boys.
"They went through a bad run but they continued to work hard.
"They've put in a terrific shift the past few weeks and have got the wee breaks to win the games.
"Football is a game of belief. If you believe in yourself and the guys round about you, you can achieve big things.
"I believe if you work hard you'll get your reward.
"Now with two in a row, we want to try to make it three."