West Ham United defender James Collins will miss Wales' Euro 2016 qualifier against Belgium in June because of a calf injury.
Collins, 31, has been ruled out of West Ham's final Premier League game of the season at Newcastle.
The former Cardiff City and Aston Villa player suffered the injury during his side's 2-1 defeat by Everton last Saturday.
Chris Coleman will name his squad for the 12 June qualifier on Tuesday.
Collins made his first appearance in Wales' Euro 2016 qualifying campaign in the 3-0 win over Israel in March.
The centre-half started in place of Hull City's James Chester, who missed the game after suffering a shoulder injury.
Chester, 26, returned to action for Hull in April but Wales will be without Tottenham left-back Ben Davies for the Belgium game.
Former Swansea City defender Davies has been ruled out after having surgery on a dislocated shoulder.
The 22-year-old defender slipped and fell awkwardly during the Premier League match away to Southampton.
Wales are currently second in Euro 2016 qualifying group B, behind Belgium on goal difference.