Conference champions Barnet have sold Underhill Stadium, which was their home for 106 years, to the government's Education Funding Agency.
The Bees left Underhill to move to The Hive in 2013, following a long-standing dispute with Barnet Council.
"Everybody will always hold dear the countless memories we have from the unforgettable years at Underhill," Bees chairman Tony Kleanthous said.
"Barnet will, of course, always be our true and traditional home."
Barnet have a 10-year agreement with Harrow Council to play at The Hive, which is also their training base.
Kleanthous says the Bees were forced to leave Underhill after relations with Barnet Council broke down.
"The final straw for me was Barnet Council's decision to give the Copthall site to another club for free, leaving us with no hope for a future stadium in the borough," Kleanthous told the club website.
"It's disappointing that local councillors were unable or unwilling to fight to keep the greatest sporting community asset this borough could have hoped for, but that's history now and we have moved on to pastures new.
"There can be no doubt that Barnet currently find themselves in a much better place than when we left Underhill in April 2013 and hope one day we will return, but [I] cannot see this in either the short or medium term."
The Education Funding Agency are expected to submit a planning application to build a school at Underhill, where Barnet first played in 1907.