Arsenal missed out on securing a Women's Champions League berth despite beating Birmingham City.
Manchester City's 2-1 win over Notts County saw them finish second in Women's Super League One and claim the last remaining European spot.
Marta Corredera and Natalia had early chances for the hosts, while Emily Westwood went closest for Blues.
Natalia met a Danielle Carter cross after the break to loop her header home, but it was not enough.
Arsenal finished three points adrift of Manchester City and miss out on Champions League football for a third successive season.
Arsenal Ladies: Van Veenendaal, Scott, Stoney, Rose, Williamson, Janssen, Losada (Sampson 81), Nobbs, Carter, Corredera (Yankey 59), Natalia (Ubogagu 59).
Substitutes not used: Byrne, Humphrey, Kelly, Bailey.
Birmingham City Ladies: Baggaley, Sargeant, Mannion, Westwood (Ayisi 73), Hallop, Carter, Potter, Allen, Carney (Wellings 68), Haines (Lawley 52), Linnett.
Substitutes not used: Scofield, Torkildsen, Peplow.
Referee: Sian Piret