Salford City's home match against Hartlepool United in the FA Cup second round will be broadcast live on BBC Two on Friday, 4 December.
The match will kick off at 19:55 GMT, with Dan Walker presenting.
Northern Premier League Premier Division side Salford, who are part-owned by five former Manchester United players, beat Notts County to reach the second round proper for the first time.
League Two Hartlepool defeated non-league Cheltenham in the first round.
"We are delighted to have the standout fixture of the second round live on BBC Two," said BBC football editor Mark Cole.
"There is clearly a real interest from the audience in Salford's progress and whether a team from the seventh tier can make it to the third round for the first time in their history."
United assistant manager Ryan Giggs, Gary and Phil Neville, Paul Scholes and Nicky Butt agreed a deal to take over Salford - who were in the eighth tier - in March 2014.
Highlights of all second-round ties will be available on the BBC Sport website, while Football Focus on 5 December will broadcast live from Northern Premier League Premier Division Stourbridge, who entertain Eastleigh, with the winners joining Premier League clubs in the third round.
Final Score on Sunday, 6 December will showcase all the goals from the day's eight ties, which are yet to be announced. The FA Cup third-round draw - one of the most eagerly awaited days in the football calendar - is live from Cardiff on Monday, 7 December.
It is the second time Salford have been chosen for live broadcast by the BBC in this season's FA Cup.
A peak audience of 3.5 million tuned in to watch Salford's first-round win over Football League opposition on BBC Two.