Plymouth Argyle manager Derek Adams says his side missed their experienced players as they lost 2-1 to local rivals Exeter City.
Luke McCormick, Peter Hartley, Carl McHugh and Reuben Reid were all missing as the Pilgrims were beaten for the third time in four games.
"You always miss experience in your team," Adams told BBC Radio Devon.
"We've got a youthful side, a lot of them are in their early 20s and it was a big occasion for them."
The Pilgrims were 2-0 down at half-time before Oscar Threlkeld got his first Argyle goal after an hour.
Threlkeld was one of four young loanees in the Argyle side, with Brighton goalkeeper Christian Walton and Crystal Palace centre-back Luke Croll making their debuts after signing on loan late last week.
"This season we've coped well with injuries and suspensions and players have come into the side and done really well," Adams added.
"Would we like to get the injuries back? Yes we would, but today we've shown we are a good side and we created a lot of good opportunities in the game and probably deserved more out of it than the loss."