France midfielder Lassana Diarra has been ruled out of Euro 2016 with a knee injury and replaced by Manchester United's Morgan Schneiderlin.
Defender Raphael Varane had already been ruled out of the host nation's campaign with a thigh injury.
The 23-year-old Real Madrid player, who has won 29 caps for Les Bleus, is out for up to three weeks.
Sevilla defender Adil Rami, 30, has been called up by manager Didier Deschamps as Varane's replacement.
France play Group A rivals Romania in the tournament opener on 10 June.
France squad for Euro 2016:
Goalkeepers: Hugo Lloris, Steve Mandanda, Benoit Costil.
Defenders: Adil Rami, Laurent Koscielny, Eliaquim Mangala, Jeremy Mathieu, Patrice Evra, Bacary Sagna, Christophe Jallet, Lucas Digne.
Midfielders: Paul Pogba, Blaise Matuidi, Morgan Schneiderlin, N'Golo Kante, Yohan Cabaye, Moussa Sissoko.
Forwards: Antoine Griezmann, Dimitri Payet, Anthony Martial, Kingsley Coman, Olivier Giroud, Andre-Pierre Gignac.