Portsmouth striker James Vaughan "got away with a punch" on Bristol Rovers defender Tom Lockyer, according to Rovers boss Graham Coughlan.
Coughlan said he was disappointed after hearing no action would be taken by the Football Association for the alleged incident during Tuesday's 1-1 draw.
"They've decided you can punch players on the football pitch and get away with it," Coughlan, who was appointed in Janaury, told BBC Radio Bristol.
The FA has said it will not comment.
"Four officials missed it at the time," Coughlan added. "The fourth official should have seen it as he is right down my eyeline and I've seen it.
"The FA have told us they won't be taking any action against what's gone on - that's fine.
"But once that happens, I hope we're not setting a precedent that it's one rule for one.
"Every proper football fan in the country will look at what I've seen and have their own thought process on what's going on.
"I guarantee every one will be thinking along the same lines as myself."
Portsmouth manager Kenny Jackett felt footage of the incident between Vaughan and Lockyer was "inconclusive" in terms of any contact or simulation between the pair.
"The player (Lockyer) has gone down and felt there's contact," he told BBC Radio Solent. "Really, it's only the two players involved who would know if there was any contact and what the level of that was.
"I'm pleased to see common sense has prevailed."
Another incident in the League One match is already being looked into by the FA after a plastic bottle was thrown from the crowd towards Lockyer at Fratton Park.