Trainer Barry Hills, who has enjoyed more than 3,200 career wins, is retiring and will hand over his licence to son Charlie.
The 74-year-old will step aside after the Ebor meeting at York next week although he will remain as managing director of the family company.
Hills began training in 1969 and won 10 Classics, including the 1,000 Guineas in 2009 with Ghanaati.
Charlie, 32, has been his assistant for eight years.
Lambourn-based Barry Hills racked up victories across the world, including the 1973 Prix de l'Arc de Triomphe with Rheingold, and broke records with Nagwa and Further Flight.
Nagwa scored 13 wins as a juvenile while Further Flight landed five Jockey Club Cups.
The trainer has overcome serious illnesses, including throat cancer, in recent years to remain at the helm of one of the biggest dynasties in horse racing.
Barry's eldest son John is an established trainer, while twins Michael and Richard are both successful jockeys.
A statement on the Hills' website said Charlie was "well groomed" for the role.
"He is thoroughly looking forward to the challenge of moving the operation at Faringdon Place forward, but realises what a formidable task he takes on in trying to emulate his father," it said.
"The owners have been tremendously supportive of the change and Charlie appreciates this hugely."