Sheikh Mohammed's Godolphin racing operation have closed down one of their smaller Newmarket stables.
Their two major bases, where Saeed bin Suroor and Charlie Appleby train, remain in the town.
Godolphin said "a relatively small number of staff" have lost jobs with the closure of Highfields Stable.
No reason has been given for the closure of the stable, which housed up to 50 horses who will be relocated around the organisation's other bases.
Godolphin have been undergoing a restructure following last year's doping scandal which saw trainer Mahmood al Zarooni banned for eight years.
There were fears at the time that the sheikh might scale back his operation following the controversy.
But the two main Newmarket stables, which have a total of around 400 horses, continue to operate although they are yet to register a Group One win at the highest level in Europe so far this season.