Ten matches take place on Saturday, with FC Halifax Town and AFC Wimbledon starting the action in a lunchtime kick-off.
Eight games follow at 15:00, including five all-League One ties.
You can watch highlights from these games on the BBC Sport website and app from 17:30 onwards, as well as follow live text commentary as the actions unfolds.
Later on Saturday, National League high-fliers Wrexham host League Two side Newport County in an all-Welsh clash at 20:00.
Eight more matches will be played on Sunday, including 2008 winners Portsmouth's trip to League One rivals Rochdale, before National League North side Guiseley meet League One team Fleetwood Town in the final tie on Monday.
There is plenty of other FA Cup action on the BBC across the weekend.
On Saturday, Football Focus on BBC One at 12:00 comes live from Maidstone's Gallagher Stadium before the National League side's tie against Oldham.
Dan Walker will be joined in Kent by former Premier League players Kevin Kilbane and Mark Schwarzer and the programme will feature plenty of FA Cup features and discussion.
BBC local radio will feature extensive coverage of the FA Cup second-round ties.
You will also be able to follow the goals as they go in on Final Score, which is available on the red button and BBC Sport website from 14:30 and on BBC One from 16:30.
On Sunday, you can watch highlights of the second-round games on BBC Two from 18:00.
The draw for the third round - when the Premier League and Championships teams enter - will be live on BBC One at 19:30 GMT on Monday from Chelsea.