Defender Geva Mentor and attacker Jo Harten will miss England's Test series against world champions Australia.
2014 World Player of the Year Mentor, who captained England in August's World Cup, Harten, Stacey Francis and Serena Guthrie will instead prepare for the domestic season.
Manchester Thunder's Sara Bayman leads a 12-strong squad including seven players not involved in the World Cup.
The three-Test series starts at Liverpool's Echo Arena on 20 January.
London's Copper Box Arena will host the final two matches on 22 and 24 January.