Head coach Brian McClennan has named nine debutants in the Exiles squad to face England on 14 June.
Mickey Paea, Brett Delaney, Chris Bailey, Michael Monaghan and Harrison Hansen are among the forwards, while Zeb Taia, Travis Burns, Michael Dobson, Rhys Hanbury bolster the backs.
St Helens' second-row Sia Soliola maintains his 100% call-up record and is set for a fourth Exiles appearance.
Warrington have four players in the squad, including
The initial squad of 25 has been trimmed, with St Helens' Willie Manu, Louis Anderson and Daryl Millard of Catalan Dragons, Huddersfield's David Faiumu, London's Antonio Kaufusi, Hull KR's Cory Paterson, Heath L'Estrange, Manese Manuokafoa and Jarrod Sammut from Bradford and Wakefield's Justin Poore omitted.
Hull FC's Mark O'Meley and Blake Green of Wigan Warriors are unavailable through injury, while 11-try Castleford winger Justin Carney is suspended.
The Exiles concept was devised to provide England with competitive warm-up fixtures and to give overseas players an opportunity for representative rugby.
McClennan coached the 2011 squad to victory in this fixture and is looking for a third win from four for the Exiles in International Origin games.
"This is a very strong squad - you've only got to look at the names of some of the players I've had to leave out to see that," he said.
"And there's no second game this year, so everything is riding on the one match. We'll be giving it our best shot and England will have to be at their very best to beat us.
"For players on either side it's their only representative opportunity before the Rugby League World Cup, so I expect a very intense battle.
"We're aiming to put on a style of rugby that does the Exiles brand proud and I really hope the Warrington public come out and support the match in huge numbers."
EXILES SQUAD TO PLAY ENGLAND
Travis Burns (Hull Kingston Rovers), Chris Bailey (London Broncos), Brett Delaney (Leeds Rhinos), Michael Dobson (Hull Kingston Rovers), Rhys Hanbury (Widnes Vikings), Harrison Hansen (Wigan Warriors), Brett Hodgson (Warrington Wolves), Lance Hohaia (St Helens), Kylie Leuluai (Leeds Rhinos), Steve Menzies (Catalans Dragons), Joel Monaghan (Warrington Wolves), Michael Monaghan (Warrington Wolves, Australia), Joel Moon (Leeds Rhinos), Mickey Paea (Hull Kingston Rovers), Tony Puletua (St Helens), Pat Richards (Wigan Warriors), Iosia Soliola (St Helens), Zeb Taia (Catalan Dragons), Trent Waterhouse (Warrington Wolves)