Wigan Warriors hooker Michael McIlorum has suffered a broken arm and will be out of action for around two months.
"We'll hopefully get him back for the play-offs," Warriors head coach Shaun Wane told BBC Radio Manchester.
"He's the best hooker in the world for me and he's going to be a loss."
Captain Sean O'Loughlin also missed the 36-28 loss at Wakefield, although he is expected to be fit to face local rivals St Helens on Friday.
Defending champions Wigan have maintained a position near the top of the table for much of the season, despite being without a number of regular first-team players because of injury.
"I've never known a season like it," added Wane. "There's not a common theme to them - they're all random injuries.
"We were top of the competition, and when you consider we've had so many players out for so long, we're in a good position. Slowly but surely, those players will be coming back."
Meanwhile, Warriors second-rower Liam Farrell is facing the threat of suspension and could miss Friday's game after being charged by the Rugby Football League for a Grade B lifting offence in the match against Wakefield.
Centre Anthony Gelling could face punishment for Grade A punching, while Bradford player-coach Matt Diskin and Hull KR's Neville Costigan have both been charged with Grade A striking.
All four players have the option to enter early guilty pleas.