Danny Brough will lead Scotland in their defence of the European Championship after turning down the chance to have a full off-season.
Ipswich Jets forward Billy McConnachie gets his first Scotland call-up.
Brough is one of five Super League players in Steve McCormack's 29-man squad.
The half-back, 32, said: "Steve is a top bloke and I enjoy playing for him. While I can keep running about and get picked, I'll continue to do that."
Brough, named this week in the Super League Dream Team after playing a key role in Huddersfield Giants' march to the semi-finals, has spent the last two autumns playing for the Bravehearts.
And he has committed to doing so again as McCormack builds towards the 2016 Four Nations Series and the 2017 World Cup.
Brough will equal Andrew Henderson's record of 21 caps if he plays in all three matches, starting with a game against Wales in Wrexham on 16 October.
McCormack, who has worked with Brough since his first training camp 11 years ago, said: "It tells you everything about the man.
"It would have been easy for him to say, 'I need a rest', but from the start of preparations for this year's European Championship, he said he wanted to play.
"Having one of the world's best half-backs leading us is superb and brings out the best in the players around him."
Wardle, Phillips and Szostak miss out
Salford hooker Liam Hood returns for the first time since winning his only cap in 2012 and Wakefield forward Jon Molloy is one of nine uncapped players in the squad.
Of those, only Australian-based McConnachie, who was man of the match in the Queensland's Intrust Super Cup Grand Final last weekend, has not been involved with Scotland before.
Hull KR prop Adam Walker and Widnes forward Ben Kavanagh, currently on loan at Wakefield, are among 12 survivors from the squad that reached the World Cup quarter-finals two years ago and among 14 survivors of last year's European title triumph.
Workington forwards Brett Phillips and Alex Szostak and Huddersfield centre Joe Wardle all miss out because of requiring operations.
Australian-based trio Kane Linnett, who plays in Sunday's NRL Grand Final for North Queensland Cowboys, Peter Wallace and Luke Douglas have been rested.
Scotland, who get together for their first training session on Sunday, play Ireland at Galashiels on 23 October before finishing their fixtures in Avignon against France on 7 November.
Scotland training squad
D Addy (Bradford), J Barlow (Swinton), S Barlow (Leigh), C Borthwick (Edinbugh Eagles), D Brough (Huddersfield, capt), H Burnett (unattached), B Carter (Workington), L Clarke (Edinburgh Eagles), S Esslemont (Hull KR), D Ferguson (Bradford), R Harris (Warrington), B Hellewell (London Broncos), L Hood (Salford), F Hutchison (Edinburgh Eagles), B Kavanagh (Widnes), C Kilday (Central Queensland Capras), B McConnachie (Ipswich Jets), J Molloy (Wakefield), F Murphy (Edinburgh Eagles), C Phillips (Workington), S Plumridge (Edinburgh Eagles), C Robertson (Edinburgh Eagles), D Scott (Doncaster), M Stringer (Sheffield Eagles), S Toal (Barrow), O Thomas (London Broncos), Adam Walker (Hull KR), Alex Walker (London Broncos), J Walker (Leigh).