Gloucester's Kingsholm stadium is set to host international rugby again when Barbarians and Ireland face each other on 29 May 2012.
The fixture is a repeat of 2008's game at the ground which Ireland won 39-14.
Barbarians commercial director Mike Burton said: "We are delighted to be back at this rugby hotbed again."
Gloucester managing director Ken Nottage added: "We've hosted a number of internationals at the stadium but a Barbarians match is always special."
He added: "It would be fantastic to get the tremendous backing of our supporters to illustrate the region's ever growing commitment to supporting the game at all levels from grass roots to the international stage."