Cornish Pirates conceded a last-minute Brendan Burke try to lose 27-26 at Bedford in the Championship play-offs.
Andrew Suniula, Matt Evans and Tom Kessell all scored first-half tries, while Rob Cook kicked seven points to help Pirates to a 26-6 half-time lead.
Alex Rae then crossed for Bedford and James Pritchard kicked two penalties.
But Pirates had Alan Paver and Carl Rimmer sin-binned late on and could not hold on as Burke scored and Pritchard put over the crucial conversion.
The defeat means the Pirates are six points behind Bedford after two play-off matches.