Scarlets will open their Pro12 campaign at home to champions Leinster on Friday 6 September in a match that will be shown by BBC Wales on Scrum V Live.
Broadcast partners S4C will show Treviso v Ospreys the next evening.
The first Welsh derby of the season will also be on Scrum V Live, when Dragons host Scarlets on 20 September.
Rugby fans will be able to see all the Welsh derby matches over Christmas and the New Year televised on either Scrum V Live or S4C's Y Clwb Rygbi.
The Welsh derbies will see the Cardiff Blues host the Dragons and the Scarlets face the Ospreys in a double-header over the festive period.
Phil Davies' Blues side will travel to face the Dragons, with new coach Lyn Jones at the helm, at Rodney Parade on Boxing Day.
The Capital City side will then host the Men of Gwent on the new artificial Arms Park pitch on New Year's Day.
The Scarlets will face the Ospreys in a double-header, with the first game at Parc y Scarlets on Boxing Day, before the return fixture on Friday, 3 January - subject to ground availability.
The Welsh regions' derby day double-header "Judgement Day" at the Millennium Stadium looks set to see the Blues play the Scarlets and the Dragons face the Ospreys on the Easter Bank Holiday weekend of 18 April, 2014.
RaboDirect PRO12 Season 2013/14
Round 1 - 6/7/8 September
Benetton Treviso v Ospreys (7 Sept)
Connacht v Zebre (tbc)
Glasgow Warriors v Cardiff Blues (6 Sept)
Munster v Edinburgh Rugby (tbc)
Newport Gwent Dragons v Ulster (6 Sept)
Scarlets v Leinster (6 Sept)
Round 2 - 13/14/15 September
Cardiff Blues v Connacht (13 Sept)
Edinburgh Rugby v Newport Gwent Dragons (tbc)
Leinster v Ospreys (14 Sept)
Scarlets v Benetton Treviso (14 Sept)
Ulster v Glasgow Warriors (tbc)
Zebre v Munster (tbc)